Informatics Publishing Limited

Gondu Eswara Rao ¹, S.A. Rahaman ², A. Prameela Rani ³, M.M. Prasada Rao ⁴

Affiliations
1 Assistant Professor, NRI College of Pharmacy, Pothavarapadu Village, Agirapalli (M) A.P.
2 Professor, Nirmala College of Pharmacy, Atmakur Village, Mangalagiri Mandal, Guntur Dt, A.P - 522 503
3 Principal & Professor, ANU College of Pharmaceutical Science, Nagarjuna Nagar, Acharya Nagarjuna University, Guntur-530 003, (A.P),
4 Assistant Professor, QIS College of Pharmacy, Vengamukkala Palem, Ongole, A.P-523272

Abstract

Chalcones were synthesized by condensing 1-[4-(1H-imidazol-1-yl) phenyl] ethanone with aromatic aldehydes derivatives in dilute ethanolic sodium hydroxide solution at room temperature according to Claisen-Schmidt condensation. All these compounds were characterized by means of their IR, 1H NMR spectroscopic data and microanalyses. The antimicrobial activity of these compounds was evaluated by the cup plate method.

Keywords

Chalcones, Synthesis, Antimicrobial activity

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S. Madhavi ¹, A. Prameela Rani ¹

Affiliations
1 University College of Pharmaceutical Sciences, Acharya Nagarjuna University, Nagarjuna Nagar, Guntur, Andhra Pradesh - 522 510, IN

Abstract

A simple and sensitive bio-analytical RP-HPLC method with PDA detection was developed and validated for the quantification of cobicistat in human plasma. Febuxostat was used as an internal standard. The analytes were extracted from human plasma samples by liquid-liquid extraction technique. Chromatographic separation was accomplished with a Column Inertsil-BDS 250(I.D-4.6mm, particle size-5μm), Mobile phase composition 0.1% OPA Buffer: Acetonitrile (45:55), Flow rate: 1 ml/min, Injection volume 50 μl, Run time 10 min, Detection wavelength 210nm, PDA detector, Column temperature 30°C. The retention time of cobicistat and internal standard was found to be 4.0min and 5.4min respectively. The calibration curve obtained was linear (r2= 0.9992) over the concentration range of 0.01-10.0 μg/ml. The proposed method was validated by performing linearity, accuracy, precision, recovery, specificity, ruggedness (precision and accuracy), stability studies, reinjection reproducibility. The results were found to be within limits. The method was validated as per the USFDA guidelines. Hence the validated method is suitable for conducting pharmacokinetic studies and therapeutic drug monitoring.

Keywords

Bio-Analytical, RP-HPLC, Cobicistat, Febuxostat, Plasma.

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P. Sivannarayana ¹, A. Prameela Rani ², V. Saikishore ³, Ch. VenuBabu ⁴, V. SriRekha ⁴

Affiliations
1 VishwaBharathi College of Pharmaceutical Sciences, Perecherla, Guntur, IN
2 College of Pharmaceutical Sciences, ANU, Guntur, IN
3 Baptla College of Pharmacy, Bapatla, Guntur (Dt), IN
4 Vishwa Bharathi College o f Pharmaceutical Sciences, Perecherla, Guntur, IN

Abstract

In vesicular drug carrier systems transfersomes (ultra-deformable carrier systems) are novel carriers, are composed of phospholipid, surfactant, water enhanced transdermal drug delivery systems(TDDS). Transfersomes are efficient in delivering the low molecular weight and as well as high molecular weight drugs through skin, consisting of hydrophobic and hydrophilic moieties together and has a result wide range of solubility. This vesicular transfersomes can deform and pass through narrow constriction (about 10 times less than their own diameter) without measurable loss, This high deformability gives better penetration of intact vesicles, and transfersomes are highly flexibility of particles, so drug targeting can be achieved by this type of delivery systems. Recently various approaches have been used to augment the transdermal delivery of bioactive, they include iontophoresis, electrophoresis, sonophorosis, chemical permeation enhancers, microneedle systems. eg: Analgesics, Anesthetic, Corticosteroids, sex Harmones, Anticancer, Insulin. transferosomes have beneficial advantages over the vesicular systems, higher stability, systemic drug release possible to other than vesicular systems.

Keywords

Transfersomes, Ultra-Deformable Carrier, Anticancer, Higher Stability, Flexibility.

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T. Neelima Rani ¹, N. K. Durga Devi ¹, A. Prameela Rani ¹, B. R. Madhavi ¹, P. S. Praveen ¹, B. S. Mrudula ¹

Affiliations
1 KVSR Siddhartha College of Pharmaceutical Sciences, Vijayawada-520010, IN

Abstract

A rapid sensitive and specific RP-HPLC method involving UV detection was developed and validated for the simultaneous determination and quantification of metoprolol and atorvastatin. Chromatography was carried out on a Zorbax X - C18 (150 X 4.6mm, 5 μm) column using buffer and acetonitrile (50: 50) as mobile phase at a flow rate of 1 ml/min and effluent was monitored at 280 nm. The assay was linear over 25 - 150 μg/ml for metoprolol and atorvastatin. The retention times of metoprolol and atorvastatin were found to be 5.265 and 6.856 min, respectively. The percentage recovery obtained was 100.8 and 100.61 % respectively.

Keywords

Metoprolol Succinate, Atorvastatin Calcium, HPLC, Combinational Dosage Form.

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Pathuri Raghuveer ¹, A. Prameela Rani ¹, Madhuri Desavatu ¹, A. Sai Chaitanya ¹, G. Srikar ¹

Affiliations
1 University College of Pharmaceutical Sciences, Acharay Nagarjuna University, Guntur-522510, IN

Abstract

Tenofovir (TF) belongs to a class of antiretroviral drugs known as nucleotide analogue reverse transcriptase inhibitors (NRTIs) used in the treatment of human immunodeficiency virus 1 (HIV-1) and hepatitis B. Despite its safety and effectiveness, TF oral administration limited by several factors : low bioavailability (25%), adverse effects like decrease in bone mineral density and severe renal adverse events, including fanconis syndrome due to frequent dosing, and high circulating plasma levels. By developing particulate drug carriers it is possible to achieve effective plasma concentration without significant fluctuation, to avoid sub-therapeutic or toxic plasma concentrations, to achieve an effective therapy with a low dosage and to reduce the dosage frequency. TF loaded floating particulate drug carriers for control release medication were designed and constructed to prolong the retention in the stomach and to facilitate drug absorption over a prolonged period of time using Extrusion ionic gelation technique employing sodium alginate alone and in combination with MC, HPMC K100M, HPMC K15M. The drug release for the optimized formulation, F10, followed first order kinetics and Higuchi plot of F10 formulation showed an R² value of 0.9582. The data were fitted to the Korsmeyer-Peppas equation and the values of diffusion exponent 'n' for the batch F10 was 0.623 which indicated the drug release by Non-Fickian diffusion, suggesting that the diffusion along with erosion/swelling plays an important role in extending the drug release. Surface morphology of microspheres was found to be Smooth. These results suggested that the TF microbeads are promising and should be investigated further in the near future as an effective oral delivery system.

Keywords

Tenofovir, Microparticulate Systems, KPMC K15M, HPMC100M.

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